The Master of Science in Physician Assistant Studies degree is conferred by the University of Kentucky Graduate School for students at the UK Lexington campus and at the rural campus located on the campus of Morehead State University. Graduates are eligible to take the Physician Assistant National Certifying Examination. After successful completion of the examination, they are eligible for state certification and licensure to practice as certified physician assistants.

In 1996, the program was extended to include instruction at Morehead State University. Now, 10 dedicated faculty members, two based at Morehead State University, and eight based in Lexington, provide instruction. Each year, an average of 40 students are admitted to the Lexington campus, and 16 to Morehead State site.

The PA program also offers international opportunities for its students including some who choose to do their clerkship rotations in various countries including Kenya, Swaziland and England. There are also other opportunities within the college to travel internationally on short-term trips to Ecuador and Mexico, with other partnerships developing as well.