Subject | Syllabus |
Biomolecules | Structure and functions; enzymes-classification, kinetics, structure, action potential, and transport processes; mechanism of action, basic concepts and designs of metabolism, Photosynthesis, respiration, Basic biology, life sciences, etc. |
Viruses | Microbial classification and diversity, Structure and classification, methods in microbiology, aerobic and anaerobic respiration, Microbial diseases, and host-pathogen interaction. |
Prokaryotic and eukaryotic cell structure | Cell cycle and cell growth control, cell signaling, signal transduction, cell-cell communication. |
Molecular structure of genes and chromosomes | Nucleic acid replication, Mutations and mutagenesis, regulatory mechanisms in prokaryotes and eukaryotes, transcription, translation, Mendelian inheritance, complementation, ; gene interaction; ETC. Extra chromosomal inheritance; microbial genetics (plasmids, transformation, transduction, conjugation); horizontal gene transfer and transposable elements; RNA interference; DNA damage and repair; chromosomal variation |
Principles of microscopy | Light, electron, fluorescent and confocal, principles of spectroscopy-UV, principles of chromatography-ion exchange, ; centrifugation-high speed and ultra; Principles of chromatography-GC, basic biology, life sciences, biotechnology and allied area: Unit 06 |
History of immunology | Innate, humoral and cell mediated immunity; antigen; antibody structure and function; molecular basis of antibody diversity; synthesis of antibody and secretion; antigen-antibody reaction; complement; primary and secondary lymphoid organ B and T cells and macrophages; major histocompatibility complex (MHC); antigen processing and presentation; polyclonal and monoclonal antibody; regulation of immune response; immune tolerance; hypersensitivity; autoimmunity; graft versus host reaction Basic biology, life sciences, biotechnology and allied area: Unit 07 |
Major bioinformatics resources and search tools | Sequence and structure databases, Molecular dynamics and simulations, sequence analysis, data mining and analytical tools for genomic and proteomic studies, etc. |
Restriction and modification enzymes | Vectors; plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; mammalian and plant expression vectors; cDNA and genomic DNA library; gene isolation, cloning and expression; transposons and gene targeting · DNA labeling; DNA sequencing; polymerase chain reactions; DNA fingerprinting; southern and northern blotting; in-situ hybridization; RAPD, RFLP; site-directed mutagenesis; gene transfer technologies; gene therapy. |
Totipotency | Regeneration of plants; plant growth regulators and elicitors; tissue culture and cell suspension culture system: Methodology, kinetics of growth and, nutrient optimization; production of secondary metabolites by plant suspension cultures · Hairy root culture; transgenic plants; plant products of industrial importance · Basic biology, life sciences, biotechnology and allied area: Unit 10 |
Animal cell culture | Media composition and growth conditions; animal cell and tissue preservation; anchorage and non-anchorage dependent cell culture; kinetics of cell growth; micro and macro carrier culture; hybridoma technology; stem cell technology; animal cloning · Transgenic animals · Basic biology, life sciences, biotechnology and allied area: Unit 11 · Chemical engineering principles applied to biological system · Principle of reactor design, ideal and non-ideal multiphase bioreactors, mass and heat transfer; Rheology of fermentation fluids, aeration and agitation; media formulation and optimization; kinetics of microbial growth · Substrate utilization and product formation; sterilization of air and media; batch, fed-batch and continuous processes; various types of microbial and enzyme reactors; instrumentation control and optimization · Unit operations in solid-liquid separation and liquid-liquid extraction; process scale-up, economics and feasibility analysis |
Engineering principle of bioprocessing | Upstream production and downstream; bioprocess design and development from lab to industrial scale; microbial, animal and plant cell culture platforms; production of biomass and primary/ secondary metabolites; biofuels, bioplastics, industrial enzymes · Antibiotics; large scale production and purification of recombinant proteins; industrial application of chromatographic and membrane based bioseparation methods; immobilization of biocatalysts (enzymes and cells) for bioconversion processes · Bioremediation-aerobic and anaerobic processes for stabilization of solid/ liquid wastes. |
Tissue culture and its application, micropropagation | - Anther and microspore culture
- Embryo and ovary culture
- In vitro germplasm conservation
- Protoplast isolation
- Protoplast fusion-somatic hybrids, cybrids
- Cryopreservation
- Organelle DNA, satellite-and repetitive DNAs
- Somaclones
|