Usually, the minimum eligibility for the course is 10+2 with subjects with a minimum of 50% marks in Physics, Chemistry and Biology. English is also a mandatory subject for eligibility in many of the universities and colleges. However, AIIMS requires an aggregate of 60% for the general category candidates and 50% for SC/ST candidates.

Also, along with these prerequisites, certain universities like Banaras Hindu University, Varanasi limits the age of the candidates ranging between 17 and 25. While, on the other hand, AIIMS has set the lower limit to be 17 years of age for any candidate to apply for the course.