Question

What is the effect of an increase in substrate concentration on an enzyme-catalyzed reaction?

• A. The rate of reaction decreases.
• B. The rate of reaction increases.
• C. The rate of reaction remains unchanged.
• D. The enzyme becomes inactive.

Hint:

Enzyme saturation occurs when all active sites are occupied by substrate molecules, making \( V_{\text{max}} \) the upper limit of the reaction rate.