Question

The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :

• A. The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
• B. The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
• C. The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics
• D. Gene ’X’ is responsible for recognitions sites and ‘Y’ is responsible for antibiotic resistance