Question:medium

Annealing process of PCR is performed at \(\_\_\_\) temperature.

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Annealing temperature in PCR depends on the melting temperature (\( T_m \)) of the primers and is typically 5°C lower than \( T_m \).
Updated On: Nov 26, 2025
  • 90-98°C.
  • 40-60°C.
  • 70-75°C.
  • 20-25°C.

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